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Title of Article

PREPARATION AND INVESTIGATION OF CYTOTOXIC PROPERTIES OF RECOMBINANT ANTI-ERBB2 IMMUNOTOXIN BASED ON PSEUDOMONAS EXOTOXIN


Issue
 
Date

Article type
 scientific article
UDC
577.27
Pages
122-126
Keywords
 


Authors
RњR°R»RµS…R°RЅRѕRІR° R•.Rђ.
RќReR¶RµRіRѕSЂRѕRґSЃRєReR№ RіRѕSЃSѓRЅReRІRµSЂSЃReS‚RµS‚ ReRј. Rќ.R?. R›RѕR±R°S‡RµRІSЃRєRѕRіRѕ

R—RґRѕR±RЅRѕRІR° Rў.Rђ.
RќReR¶RµRіRѕSЂRѕRґSЃRєReR№ RіRѕSЃSѓRЅReRІRµSЂSЃReS‚RµS‚ ReRј. Rќ.R?. R›RѕR±R°S‡RµRІSЃRєRѕRіRѕ

R‘SЂReR»RєReRЅR° Rђ.Rђ.
RќReR¶RµRіRѕSЂRѕRґSЃRєReR№ RіRѕSЃSѓRЅReRІRµSЂSЃReS‚RµS‚ ReRј. Rќ.R?. R›RѕR±R°S‡RµRІSЃRєRѕRіRѕ

REReR»SЏRіReRЅR° Rќ.R®.
RќReR¶RµRіRѕSЂRѕRґSЃRєReR№ RіRѕSЃSѓRЅReRІRµSЂSЃReS‚RµS‚ ReRј. Rќ.R?. R›RѕR±R°S‡RµRІSЃRєRѕRіRѕ

R‘R°R»R°R»R°RµRІR° R?.R’.
RќReR¶RµRіRѕSЂRѕRґSЃRєReR№ RіRѕSЃSѓRЅReRІRµSЂSЃReS‚RµS‚ ReRј. Rќ.R?. R›RѕR±R°S‡RµRІSЃRєRѕRіRѕ

R”RµRµRІ RЎ.Rњ.
RќReR¶RµRіRѕSЂRѕRґSЃRєReR№ RіRѕSЃSѓRЅReRІRµSЂSЃReS‚RµS‚ ReRј. Rќ.R?. R›RѕR±R°S‡RµRІSЃRєRѕRіRѕ


Abstract
Recombinant immunotoxin based on Pseudomonas exotoxin A fragment and antibody 4D5scFv specific to ErbB2 receptor has been expressed and purified. The immunotoxin has been shown to save the affinity of 4D5scFv for ErbB2 in the fusion protein. The cytotoxicity of the immunotoxin was estimated using cell lines expressing different levels of ErbB2. The results obtained testify to high specificity and efficacy of the immunotoxin against ErbB2-positive cancer cell lines.

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