The changes of extracellular Cl- concentration during generation of action potential (AP) induced by gradual
cooling have been investigated by the potentiometric method. The AP generation has been shown to be accompanied
by a reverse increase of Cl- concentration whose maximum corresponds to the end of AP depolarization phase.
Maximum changes of Cl- concentration in apoplast and inside excitable cells developing during AP generation have
been calculated on the basis of the results obtained. It has been shown that the Cl- equilibrium potential (at the
maximum of Cl- output) decreases approximately to the value of a membrane potential at the AP peak.
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