Вестник Нижегородского университета им. Н.И. Лобачевского. Серия: Социальные науки

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Title of Article

BDNF EFFECT ON THE FUNCTIONING OF A PRIMARY HIPPOCAMPAL CULTURE NEURON NETWORK IN GLUCOSE DEPRIVATION

Issue	2
Date	2011

Article type	scientific article	UDC	612.014:616.831.01.6-074(021)
Pages	237-242	Keywords	dissociated hippocampal culture, BDNF (brain-derived neurotrophic factor), glucose deprivation

Authors

Vedunova Mariya ValerevnaNizhegorodskiy gosuniversitet im. N.I. Lobachevskogo, Nizhegorodskaya gosudarstvennaya meditsinskaya akademiya
Sakharnova Tatyana AleksandrovnaNizhegorodskiy gosuniversitet im. N.I. Lobachevskogo, Nizhegorodskaya gosudarstvennaya meditsinskaya akademiya
Korotchenko Svetlana AleksandrovnaNizhegorodskiy gosuniversitet im. N.I. Lobachevskogo, Nizhegorodskaya gosudarstvennaya meditsinskaya akademiya
Balashova Alena NikolaevnaNizhegorodskiy gosuniversitet im. N.I. Lobachevskogo
Mukhina Irina VasilevnaNizhegorodskiy gosuniversitet im. N.I. Lobachevskogo, Nizhegorodskaya gosudarstvennaya meditsinskaya akademiya

Abstract

The effect of glucose deprivation on the functioning of a dissociated hippocampal culture neural network has been studied. We have observed an enhancement of spontaneous bioelectrical activity during short-term glucose deprivation and an abrupt irreversible decrease of bioelectrical activity, intensification of free radical oxidation and imbalance in the lactate/glucose ratio after it. Application of BDNF (1 ng/ml) in the culture has been found to reduce dramatic consequences of glucose deprivation caused by the deficiency in the energy substrate. The effect of BDNF was manifested in the normalization of the bioelectric and metabolic activity of the neural network of the primary hippocampal culture both during glucose deprivation, and in the distant recovery period.

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