Quantitative changes of pH values in higher plant stem apoplast at generation of the variation potential (VP) have been determined. A pH-sensitive fluorescent probe FITC-dextran was used for the analysis of pH dynamics. The registration of fluorescence of FITC-dextran loaded plant was carried out by the spectrofluorimetric method. It is shown that the fluorescence analysis of pH changes at VP generation has a number of advantages over potentiometric methods, in particular, the lack of inertia and the possibility of pH estimation in deep cell layers.
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