Вестник Нижегородского университета им. Н.И. Лобачевского. Серия: Социальные науки

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Title of Article

POLYMERASE CHAIN REACTION FOR DETECTION AND QUANTITATIVE COMPARISON OF SPLICING VARIANTS OF HUMAN FAS MRNA

Issue	2
Date	2012

Article type	scientific article	UDC	577.1
Pages	211-215	Keywords

Authors

Novikov Dmitriy ViktorovichNII molekulyarnoy biologii i regionalnoy ekologii NNGU im. N.I. Lobachevskogo
Lebedev Mikhail YurevichNizhegorodskiy NII travmatologii i ortopedii
Sakharnov Nikolay AleksandrovichNII molekulyarnoy biologii i regionalnoy ekologii NNGU im. N.I. Lobachevskogo
Utkin Oleg VladimirovichNII molekulyarnoy biologii i regionalnoy ekologii NNGU im. N.I. Lobachevskogo; Nizhegorodskiy NII epidemiologii i mikrobiologii im. akad. I.N. Blokhinoy
Balmusova Elena AnatolevnaNizhegorodskiy NII travmatologii i ortopedii
Alyasova Anna ValerevnaNizhegorodskaya gosudarstvennaya meditsinskaya akademiya

Abstract

A technique for detection and quantitative comparison of splicing variants of human Fas mRNA has been developed. The detection of splicing variants is based on the classical RT-PCR method. Quantitative comparison between mRNA Fas and mRNA Fas?exo6 was performed using real-time PCR in one tube.

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