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Title of Article

NANOTECHNOLOGICAL AFM-MORPHOMETRY OF BACTERIAL CELLS


Issue
2
Date
 2013

Article type
 scientific article
UDC
579.23
Pages
34-38
Keywords
atomic-force microscopy (AFM), morphometry, bacteria


Authors
Pleskova S.N.
Laboratoriya mezhdistsiplinarnogo issledovaniya Burgundskogo universiteta, Dizhon, Frantsiya

Dubrovin E.V.
Moskovskiy gosuniversitet im. M.V. Lomonosova

Golubeva I.S.
Nizhegorodskiy gosudarstvennyy tekhnicheskiy universitet im. R.E. Alekseeva

Gorshkova E.N.
Nizhegorodskiy gosuniversitet im. N.I. Lobachevskogo

Pudovkina E.E.
Nizhegorodskiy gosudarstvennyy tekhnicheskiy universitet im. R.E. Alekseeva


Abstract
An algorithm has been developed for AFM nanoscale morphometry of bacterial cells. The analysis of quantitative characteristics has revealed the dependence of bacterial cell morphological parameters on the physicochemical properties of the substrates used. Highly oriented pyrolytic graphite and glass cause minimal deformation of bacterial cells. Nanolithography techniques for surface modification have been developed that allow native bacterial cells to be studied in a liquid environment.

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