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Title of Article

PREPARATION AND INVESTIGATION OF CYTOTOXIC PROPERTIES OF RECOMBINANT ANTI-ERBB2 IMMUNOTOXIN BASED ON PSEUDOMONAS EXOTOXIN


Issue
2
Date
 2014

Article type
 scientific article
UDC
577.27
Pages
122-126
Keywords
 recombinant immunotoxin, scFv antibodies, Pseudomonas aeruginosa exotoxin A, ErbB2 receptor, cytotoxicity.


Authors
Malekhanova E.A.
Nizhegorodskiy gosuniversitet im. N.I. Lobachevskogo

Zdobnova T.A.
Nizhegorodskiy gosuniversitet im. N.I. Lobachevskogo

Brilkina A.A.
Nizhegorodskiy gosuniversitet im. N.I. Lobachevskogo

Shilyagina N.Yu.
Nizhegorodskiy gosuniversitet im. N.I. Lobachevskogo

Balalaeva I.V.
Nizhegorodskiy gosuniversitet im. N.I. Lobachevskogo

Deev S.M.
Nizhegorodskiy gosuniversitet im. N.I. Lobachevskogo


Abstract
Recombinant immunotoxin based on Pseudomonas exotoxin A fragment and antibody 4D5scFv specific to ErbB2 receptor has been expressed and purified. The immunotoxin has been shown to save the affinity of 4D5scFv for ErbB2 in the fusion protein. The cytotoxicity of the immunotoxin was estimated using cell lines expressing different levels of ErbB2. The results obtained testify to high specificity and efficacy of the immunotoxin against ErbB2-positive cancer cell lines.

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